Cleavage of Intergenetically-econstituted Eggs by Electrofusion of Nucleus and Cytoplasm and Exchange of Pronucleus from Mouse and Rat Eggs.

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Other Title
  • 電気的融合法による異属間 (マウス・ラット) の前核と細胞質の融合ならびに前核置換卵の卵割について

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Description

Embryonic reconstitution and subsequent cleavage of the reconstituted eggs were studied by interspecific fusion of mouse and rat pronucleus and cytoplasm. Afterthe fusion of pronucleus or cytoplasm from rat egg to mouse egg with or without pronucleus, reconstitution was carried out in the following four combinations byelectrofusion.<BR>(1) Mouse egg with rat pronuclei.(2) Mouse egg with a small volume of rat cytoplasm.(3) Enucleated rat egg with mouse pronuclei.(4) Enucleated mouse egg with rat pronuclei.<BR>By any combination, reconstituted eggs could be produced in approximately 80%. The rates of cleavage of the reconstituted eggs into the two-cell stage in vitro were 68.7% for group (1), 87.5% for group (2), 43.4% for group (3) and 63.3% for group (4). These rates were significantly low compared with those obtained in controls. The rates of cleavage into the four-cell stage in vitro were 6.1% for group (1), 14.3% for group (2), 18.9% for group (3) and 3.3% for group (4), respectirely. These were again significantly lower than those in controls. These results indicated that even the reconstituted embryos produced between different species such as mouse and rat, have the ability to cleave and develop in vitro.

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Details 詳細情報について

  • CRID
    1390001204340416128
  • NII Article ID
    130003839354
  • DOI
    10.14893/jmor1991.9.7
  • ISSN
    18846513
    09167625
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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