妊馬血清性性腺刺激ホルモン投与による牛の過剰排卵誘起に関する研究

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  • Studies on the induction of superovulation by the administration of pregnant mare serum gonadotropin in dairy cows.

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The present studies were carried out to examine the efficiency of 2 drugs (PMSG and FSH) on the basis of the number of recovered eggs, fertilized eggs and normal embryos in the same cows from a single farm. The endocrine aspects were also examined to control the efficiency of superovulation by PMSG. At last, a new trial was made as an additional administration of gonadotropin releasing hormone analog (GnRH A) after PMSG and PGF2 α injections for the improvement of superovulation in cattle. (1) Individual cows showed differences in the ovarian response by two drugs, and did not show significant differences in the embryo collection following successful superovulaton. It seemed to be necessary to examine the endocrine aspects of cows during experiment to gain the efficient results of superovlation by PMSG. (2) It was obvious that cows showing the significant increases in E2 level from PMSG treatment to the estrus gave more numerous ovulatory follicles and normal embryos recovered. The increases in E2 levels from ovulation to embryo collection seemed not to have an injurious influence upon the results of embryo collection. (3) An additional administration of GnRH · A after PMSG treatment followed by a single insemination at a fixed time makes it possible to recover many normal embryos, and seems to be applicable to induce effective superovulation in the cows.

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