<i>In vitro </i>occurrence of dispersal third-stage juveniles in <i>Bursaphelenchus xylophilus</i>

DOI

Bibliographic Information

Other Title
  • 平底角型培養瓶を用いた<b><i>in vitro</i></b>培養下でのマツノザイセンチュウ分散型3期幼虫の生起
  • In vitro occurrence of dispersal third-stage juveniles in Bursaphelenchus xylophilus

Search this article

Abstract

The pinewood nematode (PWN), Bursaphelenchus xylophilus, was cultured on the gray mold fungus, Botrytis cinerea, grown on potato-dextrose-agar medium (Pearlcore PDA‘Eiken') diluted 5 times and supplemented with glycerol (3%) in a flattened culture bottle (area of the base: 24 cm2). The mean number of dispersal 3rd stage juveniles (DSJ3) and the mean percentage of DSJ3 in the populations of PWN were 31,688 and 20.8% at 30 days culture. The numbers of nematodes and DSJ3 on PDA supplemented with glycerol obviously peaked higher and later than non-supplemented PDA. Dispersal 4th stage juveniles (DSJ4) developed in the culture bottles when B. xylophilus nematodes were co-incubated with pupal and adult Monochamus alternatus on the fungus. DSJ4 (mean number, 17,446) were collected from 8-day-old M. alternatus adults that had been co-incubated with B. xylophilus during pupal and adult stages. This medium supplemented with glycerol is useful for stabilizing nematode incubation and obtaining DSJ3. In addition, this method is useful to clarify the mechanism of DSJ4 induction.

Journal

Keywords

Details 詳細情報について

  • CRID
    1390001204341880192
  • NII Article ID
    130004837320
  • NII Book ID
    AA12415279
  • DOI
    10.3725/jjn.42.9
  • ISSN
    18823408
    09196765
  • Text Lang
    ja
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

Report a problem

Back to top