Role of Alloreactive Cytotoxic T Lymphocytes in Cord Blood Stem Cell Transplantation.

DOI
  • QI Jiang
    Department of Pediatrics and Clinical Immunology, Mie University School of Medicine
  • AZUMA Eiichi
    Department of Pediatrics and Clinical Immunology, Mie University School of Medicine
  • NAGAI Masataka
    Department of Pediatrics and Clinical Immunology, Mie University School of Medicine
  • HIRAYAMA Masahiro
    Department of Pediatrics and Clinical Immunology, Mie University School of Medicine
  • UMEMOTO Masakazu
    Department of Pediatrics and Clinical Immunology, Mie University School of Medicine
  • HIRATAKE Shinya
    Department of Pediatrics and Clinical Immunology, Mie University School of Medicine
  • KUMAMOTO Tadashi
    Department of Pediatrics and Clinical Immunology, Mie University School of Medicine
  • KOMADA Yoshihiro
    Department of Pediatrics and Clinical Immunology, Mie University School of Medicine
  • SAKURAI Minoru
    Department of Pediatrics and Clinical Immunology, Mie University School of Medicine

Bibliographic Information

Other Title
  • さい帯血から誘導されるアロ抗原反応性細胞傷害性Ｔ細胞のさい帯血幹細胞移植における役割

Abstract

In patients who developed graft-vs-host disease following allogeneic bone marrow transplantation, the incidence of leukemia relapse is decreased as a result of the graft-vs-leukemia (GVL) effect. In cord blood stem cell transplantation, it is not known if the GVL effect exists. We have investigated the possibility of generating cytotoxic T lymphocytes (CTL) in a mixed lymphocyte culture (MLC). The MLC was set up using cord blood mononuclear cells as responders and the irradiated mononuclear cells of a healthy adult as stimulators. After seven days, the CTL activity was measured. In 17 out of 20 cord blood samples, alloreactive CTL was generated. A simultaneous flow cytometric analysis revealed that CD8+CD45RO+ cells markedly increased following MLC. These in vitro results suggest that the GVL effect may operate in vivo in cord blood stem cell transplantation

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Keywords

Details 詳細情報について

  • CRID
    1390001204341972608
  • NII Article ID
    130004345470
  • DOI
    10.11412/jjph1987.10.426
  • ISSN
    18844723
    09138706
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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