Endophytic Dacterial Population in Philippine Sugarcane Cultivars and Isolation of Nitrogen-fixing Strains

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  • ASIS CONSTANCIO A. JR.
    School of Agriculture, Ibaraki University, Ibaraki, 300-0393 Japan
  • KUBOTA MASATSUGU
    School of Agriculture, Ibaraki University, Ibaraki, 300-0393 Japan
  • CHEBOTAR VLADIMIR K.
    All Russia-Research Institute for Agricultural Microbiology, Shosse Podbelskogo 3, St. Petersburg-Pushkin 8, 189620, Russia
  • OHTA HIROYUKI
    School of Agriculture, Ibaraki University, Ibaraki, 300-0393 Japan
  • ARIMA YASUHIRO
    Tokyo University of Agriculture and Technology, Fuchu, 183-8509 Japan
  • NISHIYAMA KOUSHI
    National Institute of Agro-Environmental Sciences, Tsukuba, 305-8602 Japan
  • TSUCHIYA KEN-ICHI
    National Institute of Agrobiological Resources, Tsukuba, 305-8602 Japan
  • AKAO SHOICHIRO
    National Institute of Agrobiological Resources, Tsukuba, 305-8602 Japan

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タイトル別名
  • Endophytic Bacterial Population in Philippine Sugarcane Cultivars and Isolation of Nitrogen-fixing Strains.

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説明

The number of endophytic bacteria in the stem of sugarcane cultivars collected from different sites in the Philippines ranged from 104 to 109 cells ml-1 juice. Bacterial population was correlated with the sugar content (% Brix) of the juice and varied depending on the cultivar and sampling site. Twenty-eight isolates had a positive reaction in the acetylene reduction assay (ARA). Presumptive tests indicated that 15 isolates were putative strains of Acetobacter diazotrophicus, 5 isolates showed similar characteristics to those of Herbaspirillum seropedicae, and 8 isolates consisted of Herbaspirillum rubribalbicans-like strains. This study confirmed the existence of N2-fixing endophytic bacteria in sugarcane cultivars in the Philippines.

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