Rapid Estimation of Population Densities of Uncultured Bacteria in the Environment.
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- WATANABE KAZUYA
- Marine Biotechnology Institute, Kamaishi Laboratories
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- HARAYAMA SHIGEAKI
- Marine Biotechnology Institute, Kamaishi Laboratories
書誌事項
- タイトル別名
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- Rapid Estimation of Population Densitie
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説明
A rapid method for estimating the population densities of uncultured bacteria in the environment was developed. This method consists of (i) addition of particular bacterial cells of a known cell number to an environmental sample, (ii) PCR amplification of partial 16S rDNA fragments with a set of eubacterial universal primers, (iii) temperature gradient gel electrophoresis (TGGE) analysis of the PCR products, and (iv) densitometry of the TGGE pattern. The utility of this competitive PCR/TGGE (cPCR/TGGE) method was demonstrated in the analysis of dominant bacterial populations in seawater sampled from the Heita sound (Kamaishi, Iwate, Japan). The nucleotide-sequence analysis of the TGGE bands revealed that the dominant populations were closely related to previously characterized uncultured marine bacteria.
収録刊行物
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- Microbes and environments
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Microbes and environments 13 (3), 123-127, 1998
日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001204344247296
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- NII論文ID
- 110001272817
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- NII書誌ID
- AA11173196
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- ISSN
- 13474405
- 13426311
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- NDL書誌ID
- 4583044
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可