Specificity of a fluorogenic DNA probe for species of a Vibrio core group.
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- SHIBA TSUNEO
- National Fisheries University
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- MURAKAMI MASATADA
- National Fisheries University
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- OHISO KAORI
- National Fisheries University
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- MATSUO YUKA
- National Fisheries University
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- MAEDA TOSHIMICHI
- National Fisheries University
Bibliographic Information
- Other Title
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- Specificity of a Fluorogenic DNA Probe
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Description
Specificity of a DNA probe developed for some species of a Vibrio core group was examined using a fluorescence resonance energy transfer system. The probe was designed based on the sequence of a 16S rDNA fragment of Vibrio parahaemolyticus cloned in a plasmid, pTPR1. The 5'-and 3'-end of the probe was ligated with fluorogenic dyes. The specificity was examined against clonal 16S rDNA fragments and chromosomal DNAs extracted from different species of Vibrio. The 16S rDNA fragments, cloned from V. parahaemolyticus, Vibrio natriegens, Vibrio fischeri, Vibrio mediterranei, and Vibrio tubiashii, had from one to four nucleotide substitutions compared with pTPR1 in the probe region. Probe hybridization with the clonal or chromosomal DNA fragments was monitored by determining the increase in fluorescence during PCR amplification. At the highest stringent condition, the probe hybridized only to identical sequences, but not to the sequences with substitution.
Journal
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- Microbes and Environments
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Microbes and Environments 13 (3), 129-135, 1998
Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles
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Details 詳細情報について
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- CRID
- 1390001204344249088
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- NII Article ID
- 110001272818
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- NII Book ID
- AA11173196
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- ISSN
- 13474405
- 13426311
- http://id.crossref.org/issn/13426311
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- NDL BIB ID
- 4583045
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed