Most Hydrocarbonoclastic Bacteria in the Total Environment are Diazotrophic, which Highlights Their Value in the Bioremediation of Hydrocarbon Contaminants

DOI Web Site PubMed 参考文献26件
  • Dashti Narjes
    Microbiology Program, Department of Biological Sciences, Faculty of Science, Kuwait University
  • Ali Nedaa
    Microbiology Program, Department of Biological Sciences, Faculty of Science, Kuwait University
  • Eliyas Mohamed
    Microbiology Program, Department of Biological Sciences, Faculty of Science, Kuwait University
  • Khanafer Majida
    Microbiology Program, Department of Biological Sciences, Faculty of Science, Kuwait University
  • Sorkhoh Naser A.
    Microbiology Program, Department of Biological Sciences, Faculty of Science, Kuwait University
  • Radwan Samir S.
    Microbiology Program, Department of Biological Sciences, Faculty of Science, Kuwait University

書誌事項

公開日
2015
資源種別
journal article
DOI
  • 10.1264/jsme2.me14090
公開者
日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境生物学会

この論文をさがす

説明

Eighty-two out of the 100 hydrocarbonoclastic bacterial species that have been already isolated from oil-contaminated Kuwaiti sites, characterized by 16S rRNA nucleotide sequencing, and preserved in our private culture collection, grew successfully in a mineral medium free of any nitrogenous compounds with oil vapor as the sole carbon source. Fifteen out of these 82 species were selected for further study based on the predominance of most of the isolates in their specific sites. All of these species tested positive for nitrogenase using the acetylene reduction reaction. They belonged to the genera Agrobacterium, Sphingomonas, and Pseudomonas from oily desert soil and Nesiotobacter, Nitratireductor, Acinetobacter, Alcanivorax, Arthrobacter, Marinobacter, Pseudoalteromonas, Vibrio, Diatzia, Mycobacterium, and Microbacterium from the Arabian/Persian Gulf water body. A PCR-DGGE-based sequencing analysis of nifH genes revealed the common occurrence of the corresponding genes among all the strains tested. The tested species also grew well and consumed crude oil effectively in NaNO3 -containing medium with and without nitrogen gas in the top space. On the other hand, these bacteria only grew and consumed crude oil in the NaNO3 -free medium when the top space gas contained nitrogen. We concluded that most hydrocarbonoclastic bacteria are diazotrophic, which allows for their wide distribution in the total environment. Therefore, these bacteria are useful for the cost-effective, environmentally friendly bioremediation of hydrocarbon contaminants.

収録刊行物

参考文献 (26)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ