Influence of protein immobilization methods on surface plasmon resonance measurement of drug-protein interaction

HASHIMOTO Takashi, FUJIWARA Kazuhiko, ITO Hideaki, OGAWA Nobuaki

doi:10.5188/jsmerj.24.20

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Other Title

表面プラズモン共鳴法による薬剤－タンパク質相互作用測定におけるセンサ表面へのタンパク質固定化状態の効果の検証
ヒョウメンプラズモンキョウメイホウニヨルヤクザイ : タンパクシツソウゴサヨウソクテイニオケルセンサヒョウメンエノタンパクシツコテイカジョウタイノコウカノケンショウ

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Abstract

An immobilization of serum albumin and warfarin-serum albumin interaction were studied by means of surface plasmon resonance measurement. Physical and chemical immobilizations of human serum albumin (HSA) and bovine serum albumin (BSA) onto sensor surface were examined to resolve how protein immobilization methods on sensor surface affect for protein-drug molecule interaction measurement. Spontaneous adsorptions of HSA and BSA onto gold surface were utilized for physical immobilization. Adsorptions of BSA and HSA were described by Langmuir-type adsorption, and the adsorptivity of BSA were higher than the one of HSA. 11-mercaptoundecanoic acid monolayer was employed as anchor for the chemical immobilization of HSA molecule. Chemically and physically immobilized HSA-warfarin interaction was examined and compared. Binding amount of warfarin to chemically immobilized HSA was larger than physically immobilized one. It is suggested that the chemical immobilization of protein molecule onto sensor surface is preferred for protein-drug molecule interaction measurements.

Journal

Journal of the Society of Materials Engineering for Resources of Japan

Journal of the Society of Materials Engineering for Resources of Japan 24 (1_2), 20-24, 2012

The Society of Materials Engineering for Resources of JAPAN

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