Comparison of Sulphate-reducing Bacterial Communities in Japanese Fish Farm Sediments with Different Levels of Organic Enrichment
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- Kondo Ryuji
- Department of Marine Bioscience, Fukui Prefectural University
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- Mori Yumi
- Department of Marine Bioscience, Fukui Prefectural University
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- Sakami Tomoko
- Tohoku National Fisheries Research Institute, Fisheries Research Agency
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Abstract
Fish farm sediments receive a large amount of organic matter from uneaten food and fecal material. This nutrient enrichment, or organic pollution, causes the accumulation of sulphide in the sediment from the action of sulphate-reducing bacteria (SRB). We investigated the effect of organic enrichment around coastal fish farms comparing the SRB community structure in these sediments. Sediment samples with different levels of organic pollution classified based upon the contents of acid-volatile sulphide and chemical oxygen demand were collected at three stations on the coast of western Japan. The SRB community composition was assessed using PCR amplification, cloning, sequencing and phylogenetic analysis of the dissimilatory sulphite reductase b-subunit gene (dsrB) fragments using directly extracted sediment DNA. Sequencing of the cloned PCR products of dsrB showed the existence of different SRB groups in the sediments. The majority of dsrB sequences were associated with the families Desulfobacteraceae and Desulfobulbaceae. Clones related to the phylum Firmicutes were also detected from all sediment samples. Statistical comparison of sequences revealed that community compositions of SRB from polluted sediments significantly differed from those of moderately polluted sediments and unpolluted sediments (LIBSHUFF, p<0.05), showing a different distribution of SRB in the fish farm sediments. There is evidence showing that the organic enrichment of sediments influences the composition of SRB communities in sediments at marine fish farms.<br>
Journal
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- Microbes and Environments
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Microbes and Environments 27 (2), 193-199, 2012
Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles
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Details 詳細情報について
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- CRID
- 1390001204346450432
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- NII Article ID
- 10030432139
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- NII Book ID
- AA11551577
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- COI
- 1:STN:280:DC%2BC38jps1KnsA%3D%3D
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- ISSN
- 13474405
- 13426311
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- NDL BIB ID
- 023727902
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- PubMed
- 22791053
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed