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Development of Culture Medium for the Isolation of <i>Flavobacterium</i> and <i>Chryseobacterium</i> from Rhizosphere Soil
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- Nishioka Tomoki
- Graduate School of Applied Biological Sciences, Gifu University
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- Elsharkawy Mohsen Mohamed
- Department of Agricultural Botany, Faculty of Agriculture, Kafrelsheikh University
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- Suga Haruhisa
- Life Science Research Center, Gifu University
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- Kageyama Koji
- River Basin Research Center, Gifu University
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- Hyakumachi Mitsuro
- Graduate School of Applied Biological Sciences, Gifu University
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- Shimizu Masafumi
- Graduate School of Applied Biological Sciences, Gifu University
Bibliographic Information
- Other Title
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- Development of Culture Medium for the Isolation of Flavobacterium and Chryseobacterium from Rhizosphere Soil
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Description
An effective medium designated phosphate separately autoclaved Reasoner’s 2A supplemented with cycloheximide and tobramycin (PSR2A-C/T) has been developed for the isolation of Flavobacterium and Chryseobacterium strains from the plant rhizosphere. It consists of Reasoner’s 2A agar (R2A) prepared by autoclaving phosphate and agar separately and supplementing with 50 mg L−1 cycloheximide and 1 mg L−1 tobramycin. A comparison was made among the following nine media: PSR2A-C/T, PSR2A-C/T supplemented with NaCl, R2A agar, R2A agar supplemented with cycloheximide and tobramycin, 1/4-strength tryptic soy agar (TSA), 1/10-strength TSA, soil-extract agar, Schaedler anaerobe agar (SAA), and SAA supplemented with gramicidin, for the recovery of Flavobacterium and Chryseobacterium strains from the Welsh onion rhizosphere. Flavobacterium strains were only isolated on PSR2A-C/T, and the recovery rate of Chryseobacterium strains was higher from PSR2A-C/T than from the eight other media. In order to confirm the effectiveness of PSR2A-C/T, bacteria were isolated from onion rhizosphere soil with this medium. Flavobacterium and Chryseobacterium strains were successfully isolated from this sample at a similar rate to that from the Welsh onion rhizosphere.
Journal
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- Microbes and Environments
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Microbes and Environments 31 (2), 104-110, 2016
Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles
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Details 詳細情報について
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- CRID
- 1390001204346754176
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- NII Article ID
- 130005158508
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- NII Book ID
- AA11551577
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- ISSN
- 13474405
- 13426311
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- NDL BIB ID
- 027435762
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- PubMed
- 27098502
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- JaLC
- NDL Search
- Crossref
- PubMed
- CiNii Articles
- KAKEN
- OpenAIRE
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- Abstract License Flag
- Disallowed
