温熱誘導型プロモーター<I>gadd</I>153による<I>TNF-α</I>遺伝子発現誘導

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  • Induction of TNF-.ALPHA. Gene Expression by Heat Inducible Promoter gadd 153.
  • Induction of TNF-α gene expression by heat inducible promoter gadd 153

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We demonstrated the effectiveness of inducible expression of the TNF-α gene. The TNF-α gene located under the heat-inducible promoter, gadd 153, was introduced into the human glioma cell line, U251-SP. Without heat treatment, no cytotoxicity for the transfected cells was observed. When the transfected cells were treated with hyperthermia at 43°C for 1 h or 45°C for 1 h, inducible expression of the TNF-α gene was observed. The pattern of enhancement of the gene expression varied with temperature. The TNF-α production per cell on treatment at 43°C reached 4.3 times the basal level at one day after the heat treatment while the maximum production on treatment at 45°C was observed at 4 days after the heating. The cytotoxicity of the transfected cells treated at 43°C and at 45°C was 81% at 3 days after the heating and 99.9% at 4 days after the heating, respectively.

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