Measurement of Methanogenic Bacteria by Fluorescence Coenzyme F420 Using Image Analysis.

IHARA Ikko, MAEKAWA Takaaki

doi:10.11449/sasj1971.30.247

Bibliographic Information

Other Title

補酵素Ｆ４２０の画像処理によるメタン菌体密度の測定
ホコウソ F420 ノガゾウショリニヨルメタンキンタイミツドノソクテイ

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Description

The fluorescence coenzyme F₄₂₀ was measured as an indicator of methane fermentation. The liquid portion from the methanogenic fermentor was irradiated at an excitation light of 420nm and observed by fluorescence microscopy. Changes in the fluorescence pixel rate were calculated by an image analyzer during the batch growth of acetotrophic and hydrogenotrophic methanogens. Correlation between the fluorescence pixel rate and the methane production ratio in both methanogens was high. This measurement can potentially help to characterize more metabolic status of the methanogenic bacteria.

Journal

Nogyo Shisetsu (Journal of the Society of Agricultural Structures, Japan)

Nogyo Shisetsu (Journal of the Society of Agricultural Structures, Japan) 30 (3), 247-256, 1999

The Society of Agricultural Structures, Japan

Keywords

Details 詳細情報について

CRID: 1390001204353539840

NII Article ID: 130004280115; 40004003585

NII Book ID: AN00201054

DOI: 10.11449/sasj1971.30.247

ISSN: 21860122; 03888517

NDL BIB ID: 4952615

Web Site: http://id.ndl.go.jp/bib/4952615; https://ndlsearch.ndl.go.jp/books/R000000004-I4952615

Text Lang: ja

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