オープンウェル型マイクロチップを用いる酵素免疫測定法の開発

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タイトル別名
  • Development of Enzyme Linked Solvent Assay on Open-Well Microchip
  • オープンウェルガタ マイクロチップ オ モチイル コウソ メンエキ ソクテイホウ ノ カイハツ

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An enzyme-linked immunosorbent assay (ELISA) on the surface of a glass open-well microchip (4 mmφ, 10 μm Depth) was developed. The analytical principle of this method for the determination of immunoglobulin A (IgA) was the same as the conventional sandwich-type ELISA. Firstly, a primary antibody (anti-IgA) was adsorbed on the surface of an open-well microchip, and then an antigen (IgA) and secondary antibody (anti-IgA HRP labeled) were reacted with the adsorbed antibody. The HRP remained on the surface of the open-well microchip, which was determined by using Amplex® Red System with an incident-light fluorescence microscope after the reacyion for 90 seconds. The calibration curve of the IgA standard solution was linear in the range up to 5 ng/ml on 10 μl of the substrate volume. The proposed method with the openwell microchip shortened the reaction time compared to the conventional ELISA with a 96-well plate.<br>

収録刊行物

  • 分析化学

    分析化学 55 (5), 313-316, 2006

    公益社団法人 日本分析化学会

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