Simultaneous Determination of Residual Veterinary Drugs in Muscle by On-Line Solid Phase Extraction/High-Performance Liquid Chromatography/Tandem Mass Spectrometry

TAGIRI-ENDO Misako, YANAGITA Noriaki

doi:10.2116/bunsekikagaku.56.317

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オンラインカラム固相抽出／高速液体クロマトグラフィー／タンデム質量分析法による筋肉中残留動物用医薬品の一斉分析
オンラインカラムコソウチュウシュツコウソクエキタイクロマトグラフィータンデムシツリョウブンセキホウニヨルキンニクチュウザンリュウドウブツヨウイヤクヒンノイッセイブンセキ

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A rapid simultaneous assay was developed for determining 63 veterinary drugs in bovine, porcine, and poultry muscles by on-line solid phase extraction (on-line SPE)/high-performance liquid chromatography/tandem mass spectrometry (MS/MS). The drugs were extracted from muscle samples with acetonitrile-methanol (4 : 1, v/v). The extracts were evaporated, and the residues were dissolved with acetonitrile-water (1 : 1, v/v). The samples were injected into an on-line SPE column packed with Oasis HLB using 0.1% formic acid as a mobile phase, to separate drugs from interfering substances and retain them on it. The drugs were introduced onto an analytical column using 0.1% formic acid- acetonitrile in a gradient mode by column switching and directed into MS/MS. Under the optimal analytical conditions, the repeatability of the retention time and the peak area of standard compounds were less than 1% and 12%, respectively. The recoveries of 63 drugs from muscle spiked at 20 ng/g were 45.8∼153%. The detection limits were less than or equal to10 ng/g.<br>

Journal

BUNSEKI KAGAKU

BUNSEKI KAGAKU 56 (5), 317-326, 2007

The Japan Society for Analytical Chemistry

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