Selective Separation of Diphenylarsinic Acid in Biological Samples by Combined Solvent Extraction with Solid-Phase Extraction and Determination by Graphite-Furnace Atomic-Absorption Spectrometry

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  • UENO Seiichi
    Ibaraki Prefectural Institute of Public Health
  • KITAMURA Tatsumi
    Ibaraki Prefectural Institute of Public Health Present address, Ibaraki Kasumigaura Environmental Science Center
  • NAKAMURA Miki
    Ibaraki Prefectural Institute of Public Health
  • OZONE Keiko
    Ibaraki Prefectural Institute of Public Health
  • ISHIZAKI Mutsuo
    Ibaraki Prefectural Institute of Public Health Present address, Public Health Reseach Center of Ibaraki Pharmaceutical Association

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Other Title
  • 溶媒抽出及び固相抽出法を用いる生体試料中のジフェニルアルシン酸の選択的分離法と黒鉛炉原子吸光法による定量
  • ヨウバイ チュウシュツ オヨビコソウ チュウシュツホウ オ モチイル セイタイ シリョウ チュウ ノ ジフェニルアルシンサン ノ センタクテキ ブンリホウ ト コクエンロ ゲンシ キュウコウホウ ニ ヨル テイリョウ

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Abstract

Diphenylarsinic acid (DPAA) was extracted selectivity and efficiently from biological samples by a combined solvent extraction with solid-phase extraction and was determined by graphite-furnace atomic-absorption spectrometry (GFAAS). After 0.01∼0.1 g of biological samples were decomposed in 1 ml of 2 M sodium hydroxide by heating (90∼120°C, 2∼5 h), 1 ml of water was added. The decomposed solution was shaked for 5 min with 1 ml of chloroform and 4 ml of n -hexane, and the organic layer was discarded. The aqueous layer was adjusted to ca. 0.5 M hydrochloric acid solution by the addition of 2 ml of 2 M hydrochloric acid, and 1 ml of 20% cysteine solution and 1 ml of 40% potassium iodide solution were added to this solution. After 30 minutes, DPAA was extracted with 4 ml of chloroform. This operation was repeated again and combined chloroform layers were evaporated. After the residue was dissolved in 0.5 ml of ethanol, 10 ml of a 1% nitric acid solution was added and mixed well. The mixture was then passed through a 0.45 μm of membranefilter and 0.2 ml of a 0.05 M EDTA solution was added to the filtrate. The solution was applied on an Oasis HLB cartridge and DPAA was eluted in 6 ml of ethanol. After evaporation of the solvent, the residue was dissolved in 1 ml of water and DPAA was analyzed by GFAAS. The proposed method could determine DPAA in biological samples, such as mouse brain, liver and kidney with an average recovery of 93%. The coefficient of variation was as low as 15%. The calibration curve was linear between 0 and 50 ng/ml (r = 0.999).<br>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 55 (1), 9-13, 2006

    The Japan Society for Analytical Chemistry

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