Determination of Histamine in Fish Sauce by Photometric Flow Injection Analysis with Immobilized Histamine Oxidase Reactor

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  • Watanabe Shun
    Department of Applied Chemistry and Biotechnology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi
  • Matsuo Kenichi
    Department of Applied Bioscience, Faculty of Engineering, Kanagawa Institute of Technology
  • Suzuki Yasutada
    Department of Applied Chemistry and Biotechnology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi
  • Tachibana Masaki
    Department of Applied Chemistry and Biotechnology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi
  • Tani Kazue
    Department of Applied Chemistry and Biotechnology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi
  • Koizumi Hitoshi
    Department of Applied Chemistry and Biotechnology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi
  • Matsumoto Kunio
    Department of Applied Bioscience, Faculty of Engineering, Kanagawa Institute of Technology
  • Kiba Nobutoshi
    Department of Applied Chemistry and Biotechnology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi

Bibliographic Information

Other Title
  • 固定化ヒスタミンオキシダーゼを用いる吸光検出フローインジェクション分析による魚醤中のヒスタミンの定量
  • コテイカ ヒスタミン オキシダーゼ オ モチイル キュウコウ ケンシュツ フロー インジェクション ブンセキ ニ ヨル ギョショウチュウ ノ ヒスタミン ノ テイリョウ

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Abstract

Histamine in fish sauce was rapidly determined with a single-line flow-injection system having an immobilized histamine oxidase/peroxidase reactor. Hydrogen peroxide produced by histamine oxidase (EC1.4.3.-) reaction was monitored photometrically by using N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methoxyaniline and 4-aminoantipyrine as color reagents with peroxidase (EC 1.11.1.7) at 530 nm. The carrier solution was 0.1 M CHES-NaOH buffer (pH 8.0). The reactor was stable for 2 weeks. The detection limit was 3 μM and the range of determination was 10∼1000 μM. The sample throughput was about 20/h without any carryover. The results showed a good correlation with those obtained using a conventional HPLC method.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 56 (12), 1033-1036, 2007

    The Japan Society for Analytical Chemistry

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