Manipulation of a Single DNA Molecule under the Pin-Fiber Video Scope

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Other Title
  • ピンファイバービデオスコープによるＤＮＡ一分子操作
  • ピンファイバービデオスコープ ニ ヨル DNA 1ブンシ ソウサ

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Abstract

A micro spot illumination for a single molecule was achieved by using a single optical fiber with a laser source under a microscope (pin-fiber video-scopic method). This system is suitable for observing the microscopic kinetics of colloidal particles, living cells and DNA molecules. In this paper, we discuss investigations of the DNA extension scheme and its photocleavage. Under optimum conditions, DNA twisted movements, followed by cleavage, were observed when the DNA molecule was intensely irradiated by laser radiation via a single optical fiber. It was suggested that DNA chain vibration is caused by thermal-energy transformation of the absorbed illumination; the following cleavage is achieved by active oxygen in the solution.<br>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 54 (6), 455-458, 2005

    The Japan Society for Analytical Chemistry

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