Supramolecular Assembly of Fullerene Derivatives in the Absence or Presence of Double Stranded DNA in Water

NOJIMA Takahiko, YAMASHITA Kenichi, HATTA Taizo, TSUGE Otohiko, IWASE Toshio, MAKITA Naoko, YOSHIKAWA Kenichi, FUJII Satoshi, TAKENAKA Shigeori

doi:10.2116/bunsekikagaku.54.449

The physicochemical behaviors of cationic water-soluble fullerene derivatives 1a-c (alpha-, beta-, and gamma-isomers, respectively) were studied in the absence or presence of bacteriophage T4 DNA as a double-stranded DNA (dsDNA). In water, 1a and 1b formed similar assemblies with diameters of 330 ~ 340 nm, identified by dynamic light scattering (DLS) measurements, whereas 1c formed a larger assembly with a diameter of 1400 nm. DLS and fluorescence microscopic studies showed that these assemblies collapsed after the addition of dsDNA to form new assemblies with dsDNA. In these assemble, 1a-c were formed to be lying in the DNA grooves with stoichiometric ratio of their cationic site to the DNA phosphate anionic site by spectroscopic and Transmittion electron microscopic measurements. A molecular-dynamic calculation of their complex revealed that fullerene moiety of 1a-c interacts with the groove surface of dsDNA while keeping the electrostatic interaction of the cation of 1a-c with the DNA phosphate anion. The results thus obtained have a potential for use in the new nano-structure assembly by using a dsDNA backbone.<br>

Supramolecular Assembly of Fullerene Derivatives in the Absence or Presence of Double Stranded DNA in Water

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