透析除たんぱく法による血しょう中グルクロン酸の定量

田村 善蔵, 中島 暉躬, 田中 弥恵, 三宅 聡子

doi:10.2116/bunsekikagaku.11.1279

透析除たんぱく法による血しょう中グルクロン酸の定量

DOI Web Site Web Site

田村善蔵

東京大学薬学部
中島暉躬

東京大学薬学部
田中弥恵

東京大学薬学部
三宅聡子

東京大学薬学部

書誌事項

タイトル別名

Determination of glucuronic acid in blood plasma by dialysis.
トウセキジョタンパクホウニヨルケッショウチュウグルクロンサンノテイリョウ

この論文をさがす

抄録

In the determination of glucuronic acid in blood plasma, dialysis was employed for deproteinization to avoid any coprecipitation or absorption of glucuronic acid with protein in the conventional methods. Blood plasma diluted two fold with Ringer solution (0.18ml) was dialysed against equal volume of distilled water using the dialyser in Fig. 1 and was set for 5.5 hours in an ice box. Glucuronic acid was determined on the dialysate by naphthoresorcinol method with a slight modification where the colorimetric procedure was reduced in its magnitude to one eighth. Determinations on blood samples added with known amounts of glucuronic acid resulted in recovery rate of 95105%.