インドロ-α-ピロンの生成によるインドール-3-酢酸のけい光分析

DOI NDLデジタルコレクション Web Site Web Site

書誌事項

タイトル別名
  • インドロ‐α‐ピロンの生成によるインドール‐３‐酢酸のけい光分析
  • Fluorometric determination of indole-3-acetic acid (IAA) by the formation of indolo-α-pyrone
  • インドローアルファ ピロン ノ セイセイ ニヨル インドールー3 サクサン ノ

この論文をさがす

説明

Indole-α-pyrone was used by A. Stoessl and M. A.Venis for the purpose of fluorometric determination of IAA.<BR>In this work, analytical conditions have been studied to improve their method. The following analytical procedure is recommended : An aqueous solution of 1 ml contained 5× 10-51 × 10-7 mol/l of IAA is taken in a test tube and dried in a vacuum desiccator at room temperature. Then 0.5 ml of mixture (1 + 1 by vol.) of caproic anhydride and boron trifluoride (BTF) is added into the tube. After standing for 15 min. at 0°C, 5 ml of KHCO3(5% W/V) solution is added. After shaking, indolo-α-pyrone formed is extracted with 5 ml of n-butanol. The fluorescence is measured at 500 nm by using an excitation at 440 nm. By the proposed method, for intensity of the fluorescence is remarkably enhanced. This reaction depends on the catalytic action of Lewis acid. Interference by diverse indole compounds and inorganic ions was studied in mole ratio range from 10 to 100 times against 1 ml of IAA (5 × 10-7 mol/l). In the determination of IAA, many indole compounds except 5-hydroxy-IAA and indole-3-acrylic acid did not interfere.

収録刊行物

  • 分析化学

    分析化学 24 (10), 661-663, 1975

    公益社団法人 日本分析化学会

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ