Spectrophotometric Multi-Sample Determination for Trace Amounts of Hemoglobin Using Microtiter Plate Based on the Oxidative Decomposition of the Copper(II)-Phthalocyanine Complex

HORIGUCHI Takahide, MANAKA Atsushi, KUBOTA Toshio, IGARASHI Shukuro

doi:10.2116/bunsekikagaku.54.885

Bibliographic Information

Other Title

銅‐フタロシアニン錯体の酸化分解反応を用いる微量ヘモグロビンのマイクロプレート吸光光度定量
銅-フタロシアニン錯体の酸化分解反応を用いる微量ヘモグロビンのマイクロプレート吸光光度定量
ドウフタロシアニンサクタイノサンカブンカイハンノウオモチイルビリョウヘモグロビンノマイクロプレートキュウコウコウドテイリョウ

Search this article

Description

Spectrophotometric multi-sample determinations for small amounts of hemoglobin were performed using a 96-well microtiter plate based on an oxidative decomposition reaction of the copper(II)-phthalocyanine complex. As a result of examining the optimization of various conditions, the concentration of peroxomonosulfate as an oxidizing agent was 3.6 × 10⁻² M, the pH was 2.0, and the concentration of sodium sulfate as a salt, which was contained in the pH buffer solution, was about 6.5 × 10⁻² M. In the proposed method, the calibration range was 30∼258 ng/ml. The detection limits (3 σ) were 16.99 ng/ml, and the relative standard deviation for 96 samples was 3.64% at the midpoint of the calibration range. As a result of examining foreign substances, cobalt(II), iron(III) and albumin had some influence, but other metal ions and organic compounds did not have any effect. The proposed method was successfully applied to the detection of trace amounts of hemoglobin in human serum samples. Moreover, the hemoglobin analysis for 96 samples was possible to be simultaneously performed within about ten minutes, including not only the time spent for the quantitative operation, but also for the measurement time.<br>

Journal

BUNSEKI KAGAKU

BUNSEKI KAGAKU 54 (9), 885-889, 2005

The Japan Society for Analytical Chemistry

Keywords

Details 詳細情報について

Export

Report a problem