Observation of Lipid Bilayer Phase Transition Using Ion Sensor

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  • YAMASAKI Naho
    Advanced Course in Materials Science and Engineering, Kochi National College of Technology
  • MATSUOKA Kazuhiko
    Advanced Course in Materials Science and Engineering, Kochi National College of Technology
  • NAGAYAMA Kazuhito
    Department of Materials Science and Engineering, Kochi National College of Technology
  • HATA Takashi
    Department of Materials Science and Engineering, Kochi National College of Technology
  • MATSUKI Hitoshi
    Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima
  • SATAKE Hiromu
    Center for Cooperative Research, The University of Tokushima
  • KANESHINA Shoji
    Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima

Bibliographic Information

Other Title
  • イオン選択電極法による脂質二分子膜の相転移観測
  • アナリティカルレポート イオン選択電極法による脂質二分子膜の相転移観測
  • アナリティカルレポート イオン センタク デンキョクホウ ニ ヨル シシツ 2ブンシ マク ノ ソウ テンイ カンソク

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Abstract

Some aromatic amine local anesthetics are a kind of amphiphilic ion. Binding of local anesthetics, dibucaine (DC), bupivacaine (BC), and lidocaine (LC) to a dipalmitoylphosphatidylcholine (DPPC) bilayer membrane was studied by potentiometry with ion-selective electrodes sensitive to DC, BC and LC cations, respectively. The DPPC membrane-buffer partition coefficient was directly determined as a function of the anesthetic concentration and the temperature. The DPPC bilayer underwent a thermotropic pretransition from the lamellar gel phase to the ripple gel phase as well as a main transition from the ripple gel phase to the liquid crystal phase. The partition coefficients of anesthetics at pH 5.5 varied with the temperature, namely the bilayer phases. Potentiometry using an ion-selective electrode sensitive to amphiphilic ions is effective for the detection of a bilayer phase transition through partition coefficients.<br>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 54 (10), 975-978, 2005

    The Japan Society for Analytical Chemistry

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