Study based on the chemical equilibrium concerning characteristics of the determination for serum albumin by the dye-binding method based on measuring the decreased absorbance

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  • SUZUKI Yuji
    Department of Medical Technology, Saitama Prefectural University Junior College

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  • 吸光度低下度の測定に基づく色素結合法による血清アルブミン定量法の特性に関する化学平衡論的考察
  • キュウコウド テイカド ノ ソクテイ ニ モトヅク シキソ ケツゴウホウ ニ ヨル ケッセイ アルブミン テイリョウホウ ノ トクセイ ニ カンスル カガク ヘイコウロンテキ コウサツ

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Abstract

The dye-binding method based on the protein error of a pH indicator are classified into two groups: one is a methodology that records the absorbance increase in the reaction occurring at a lower pH range; the other is a methodology that records the absorbance decrease in the reaction occurring at a higher pH range. Because the procedure of the latter is somewhat troublesome compared with the former, the latter is almost not employed as the principle for determining serum protein. However, at present, with progress for an automatic analyzer, such a methodology whose procedure is somewhat troublesome is also able to be used as a principle for protein determination. Thus, since this methodology has so far only been examined a little experimentally, but not so much theoretically, the author investigated the characteristics of this methodology by means of a theoretical analysis based on the chemical equilibrium of the protein error observed in a reaction between the protein and a pH indicator, and compared these results with those obtained in an experiment using four sulfonphthalein pH indicators. In this methodology: 1) An absorbance decrease occurs in all dyes in a restricted pH range, and its magnitude changes by an equilibrium constant of the reaction, dye concentration, and molar absorptivities of the dye and the dye-protein complex. 2) The decreased absorbance observed in the reaction is proportional to the protein concentration; this phenomenon is applicable to the determination of serum albumin. 3) As the dye concentration increases, the calibration curve approaches a straight line. These calculated results agree well with the experimental results, indicating that the calculation described here is useful as a tool to examine the characteristics of the dye-binding method.<br>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 53 (7), 691-698, 2004

    The Japan Society for Analytical Chemistry

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