Coloration of Protein Based on the Selective Reaction of Tryptophan Residues and Aldehydes

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  • アルデヒドとトリプトファン残基の選択的反応を利用するタンパク質の着色法
  • アルデヒド ト トリプトファン ザンキ ノ センタクテキ ハンノウ オ リヨウスル タンパクシツ ノ チャクショクホウ

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Abstract

Coloration of protein employing the reaction of a tryptophan residue and an aldehyde was investigated. It was observed that the reaction of lysozyme with 4-(N,N -dimethylamino)benzaldehyde resulted in formation of a bluish product due to formation of a chromophore group. The coloring depends on chemical structure of aldehydes. Electrophoresis of colored lysozyme using a polyacrylamide gel showed molecular weight bands corresponding to a monomer, a dimmer, and a trimer. The production of an oligomer was also confirmed by MALDI TOF-MS spectroscopy. Further, it is suggested that the present procedure is applicable to transcription of protein on a membrane by a western blotting technique.<br>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 56 (7), 567-572, 2007

    The Japan Society for Analytical Chemistry

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