Luminescence of Cup-Stacked Carbon Nanotubes and Its Application to Microchip Electrophoresis
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- Inoue Yousuke
- Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
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- Okamoto Yukihiro
- Department of Applied Chemistry, Graduate School of Engineering, Nagoya University MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University
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- Kaji Noritada
- Department of Applied Chemistry, Graduate School of Engineering, Nagoya University MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University
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- Tokeshi Manabu
- Department of Applied Chemistry, Graduate School of Engineering, Nagoya University MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University
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- Baba Yoshinobu
- Department of Applied Chemistry, Graduate School of Engineering, Nagoya University MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University Plasma Nanotechnology Research Center, Nagoya University Health Technology Reseach Center, AIST Institute for Molecular Science
Bibliographic Information
- Other Title
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- カップ積層型カーボンナノチューブの発光現象とマイクロチップ電気泳動への応用
- カップ セキソウガタ カーボンナノチューブ ノ ハッコウ ゲンショウ ト マイクロチップ デンキ エイドウ エ ノ オウヨウ
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Abstract
Microchip electrophoresis (MCE) is one of the most suitable methods for biomolecular separation and analysis because of its superior characteristics. In biomolecular separation and analysis, laser induced fluorescent (LIF) detection is frequently employed in MCE and attains high sensitive detection. However, LIF detection requires the labeling of analytes, which contains troublesome procedures and results in decreasing of the separation efficiency. In addition, the labeling reaction at low concentration is significantly difficult, and thus true high sensitive detection is not easily attained and has been desired. Here, we report on the possibility of cup-stacked carbon nanotubes (CSCNTs) for high sensitive label-free detection and high separation efficiency. At first we investigated fluorescence properties of CSCNTs. Our investigation revealed that supernatant solution of CSCNTs after centrifugation had fluorescence at around 500 nm, while CSCNTs suspension did not. The application of CSCNTs for MCE enabled us to successfully separate and detect DNA without labeling it. Compared to the direct detection method, our method resulted in poor separation efficiency. However, by controlling of the CSCNTs' aspect ratio and the immobilization of functional molecules on the CSCNTs surfaces, we could improve separation efficiency and attain the separation of various samples without labeling samples.
Journal
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- BUNSEKI KAGAKU
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BUNSEKI KAGAKU 58 (6), 517-521, 2009
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390001204356462720
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- NII Article ID
- 10025979400
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- NII Book ID
- AN00222633
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- COI
- 1:CAS:528:DC%2BD1MXnsl2lsL8%3D
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- HANDLE
- 2115/71684
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- NDL BIB ID
- 10336476
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- ISSN
- 05251931
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed