Evaluation of umu Test using Chemiluminescence.

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  • Evaluation of umu Test using Chemilumin

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A new type of umu test using chemiluminescence detection was developed. This method is made up of measurement of chemiluminescence which is generated from the glucose yielded as a enzyme reaction product of β-galactosidase, after several reaction steps. Salmonella typhimur-ium TA1535/pSK1002 and lactose was used as the tester strain and the substrate to measure β-galactosidase activity as an index of mutagenicity, respectively. This method was as sensitive as the fluorescence assay previously reported, and was also effective for detection of promutagens.

収録刊行物

  • 環境化学

    環境化学 6 (2), 211-215, 1996

    一般社団法人 日本環境化学会

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