Basic Research on Developing Scallop Tissue Reference Material for Quality Assurance of Diarrhetic Shellfish Poisoning (DSP) Mouse Bioassay (MBA):-Free Fatty Acid (FFA) in Homogenized Frozen Scallop Slurry and its Effect on MBA-

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  • Basic Research on Developing Scallop Tissue Reference Material for Quality Assurance of Diarrhetic Shellfish Poisoning (DSP) Mouse Bioassay (MBA): -Free Fatty Acid (FFA) in Homogenized Frozen Scallop Slurry and its Effect on MBA-

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Abstract

Diarrhetic shellfish poisoning (DSP) is one of the gastrointestinal illness caused by the consumption of shellfish contaminated with toxigenic dinoflagellates. The main toxins responsible for DSP are Okadaic acid (OA) and its derivatives. Remarkable increase of free fatty acid (FFA) in the hepatopancreas (HP) of scallops during storage in a freezer is occasionally observed and it results in pseudo-positive with the MBA for DSP. In the process of making reference material(RM) for MBA, which is consisted of a set of a vial containing a piece of filter infused with OA and DSP negative slurry of homogenized scallop whole meat (WH), we investigated the concentration of FFA. The determination of OA and FFA concentrations was performed using liquid chromatography with a fluorometric detector for anthryl diazomethane (ADAM) derivatives. In this study FFA composition and toxicity were surveyed in homogenized scallop tissue stored in a freezer at -70°C for 4 months. Most of the samples were nontoxic as determined by mouse bioassay and showed low FFA concentration; one sample showed both toxic and high FFA concentrations. These results suggest that the determination of FFA concentration in scallop tissue by HPLC coupled with the MBA for DSP is important for RM.

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