New methods for isolation of keratolytic bacteria inducing intractable hoof wall cavity (Gidoh) in a horse : double screening procedures of the horn powder agar-translucency test and horn zymography

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  • KUWANO Atsutoshi
    Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan Current affiliation: Japan Farriery Association, Tokyo 105-0004, Japan
  • NIWA Hidekazu
    Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan
  • ARAI Katsuhiko
    Department of Tissue Physiology, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan

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  • New methods for isolation of keratolytic bacteria inducing intractable hoof wall cavity (<i>Gidoh</i>) in a horse; double screening procedures of the horn powder agar-translucency test and horn zymography

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<p>To establish a new system to isolate keratolytic bacteria from the hoof wall cavity (Gidoh) of a racehorse, we invented the horn powder agar-translucency (HoPAT) test and horn zymography (HZ). Using routine bacteriological techniques and these methods, we isolated five strains of keratolytic soil bacteria, which were then identified by means of 16S ribosomal RNA (rRNA) gene sequencing analysis. The findings from the study on the horse suggested that Brevibacterium luteolum played the main role in the local fragility of the hoof, eventually forming a Gidoh in coordination with four other strains of keratolytic bacteria. The double screening procedures of the HoPAT test and HZ were useful and easy techniques for isolating the keratolytic bacteria from the horn lesions.</p>

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