Simultaneous Determination of Nonylphenol Polyethoxylates and their Biodegradation Products by High-Performance Liquid Chromatography with Fluorophotometric Detection

SATO Gaku, SAWAI Jun, KIKUCHI Mikio

doi:10.5985/jec.14.519

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Other Title

高速液体クロマトグラフ／蛍光検出法によるノニルフェノールエトキシレートおよびその生分解生成物の一斉分析
コウソクエキタイクロマトグラフケイコウケンシュツホウニヨルノニルフェノールエトキシレートオヨビソノセイブンカイセイセイブツノイッセイブンセキ

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Abstract

The widespread use of and the water pollution by nonylphenol polyethoxylates (NPnEs) have shown the need for the development of analytical procedure of NPnEs and their biodegradation products, nonylphenol polyethoxy acetic acids (NPnECs) . A method has been developed to isolate, concentrate and determine trace levels of NPnEs and NPnECs from river water samples. They were extracted with Bond Elut C18 reversed-phase minicolumn and quantified by high-performance liquid chromatography (HPLC) with fluorophotometric detection using Inertsil Ph column and eluent of 5 mM-CH₃OOONH₄in CH₃OH/H₂O (65/35) . This method can determine trace levels of each homologue of NPnEs and NPnECs, simultaneously, simply, rapidly and sensitively and minimize the risk of contamination. This method showed the limit of determination for each homologue of approx. 0.2-1.8μg/l the recovery for NPnEs and NPnECs of 84-95% at 10μg/l and the reproduceability of 2-6% of relative standard deviation for five-replicated analysis.<BR>This proposed method was employed for the determination of NPnEs and NPnECs in river water samples in Kanagawa and Tokyo, Japan. Concentration ranges between tr and 9.9μg/l for NPnECs and between nd and 5.1μg/l for NPnEs were found in Kanagawa. The high concentrations of NPnEs and NPnECs were detected in the waters sampled in 1990s in Tokyo.

Journal

Journal of Environmental Chemistry

Journal of Environmental Chemistry 14 (3), 519-529, 2004

Japan Society for Environmental Chemistry

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