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- Tarentino A.L.
- New York State Department of Health, Division of Clinical Sciences, Wadsworth Center for Laboratories and Research
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- Plummer Jr T.H.
- New York State Department of Health, Division of Clinical Sciences, Wadsworth Center for Laboratories and Research
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- 中川 裕章
- 株式会社中埜酢店・中央研究所
書誌事項
- タイトル別名
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- Deglycosylation of Asparagine-linked Glycans by PNGase F.
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説明
Peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidase F (PNGase F) is an amidase/amidohydrolase that hydrolyzes the β-aspartylglycosylamine bond of asparagine-linked glycopeptides and glycoproteins. The enzyme recognizes mainly the polypeptide region around the glycosylation site and the inner core of the carbohydrate chain. All classes of oligosaccharides are released by PNGase F, making it the enzyme of choice for deglycosylation of most asparagine-linked glycans. Used in conjunction with current biotechnology, PNGase F is an important tool for structure/function analysis of glycoproteins, structural analysis of oligosaccharides, and biosynthetic studies of asparagine-linked oligosaccharides. PNGase F is available from several commercial sources, but is relatively expensive. Alternatively, the enzyme can be purified in large amounts by most laboratories with minimal work using the one-step protocol described herein.
収録刊行物
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- Ｔｒｅｎｄｓ ｉｎ Ｇｌｙｃｏｓｃｉｅｎｃｅ ａｎｄ Ｇｌｙｃｏｔｅｃｈｎｏｌｏｇｙ
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Ｔｒｅｎｄｓ ｉｎ Ｇｌｙｃｏｓｃｉｅｎｃｅ ａｎｄ Ｇｌｙｃｏｔｅｃｈｎｏｌｏｇｙ 5 (23), 163-170, 1993
FCCA(Forum: Carbohydrates Coming of Age)
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詳細情報 詳細情報について
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- CRID
- 1390001204370485120
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- NII論文ID
- 130003699150
- 80007042721
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- NII書誌ID
- AA10995236
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- COI
- 1:CAS:528:DyaK3sXltleru7w%3D
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- ISSN
- 18832113
- 09157352
- http://id.crossref.org/issn/09157352
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
