Kinetic Analysis of Cellobiohydrolase: Quantification of Enzymatic Reaction at a Solid/Liquid Interface Applying the Concept of Surface Density

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  • Igarashi Kiyohiko
    Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Wada Masahisa
    Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Samejima Masahiro
    Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo

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Other Title
  • セロビオヒドロラーゼの反応速度論的解析：固液界面における酵素反応を表面密度という概念を用いて定量化する
  • セロビオヒドロラーゼの反応速度論的解析--固液界面における酵素反応を表面密度という概念を用いて定量化する[含 英語文]
  • セロビオヒドロラーゼ ノ ハンノウ ソクドロンテキ カイセキ コエキ カイメン ニ オケル コウソ ハンノウ オ ヒョウメン ミツド ト イウ ガイネン オ モチイテ テイリョウカ スル ガン エイゴブン

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In nature, crystalline cellulose is mainly degraded by cellobiohydrolases (CBHs) produced by various microorganisms. Since both substrate and enzymatic features relate to the reaction and the reaction proceeds at a solid/liquid interface, it is quite difficult to perform the quantitative analysis of CBH. In the present study, we estimate the surface area of crystalline celluloses by the adsorption maxima (Amax) of CBH, the specific activity of CBH (k=v/A) was plotted versus surface density (ρ=A/Amax) to adjust for the difference of surface area, and quantify the reaction rate of CBH. The results obtained from the approach indicate that the feature of crystalline cellulose greatly affects the reaction of CBH.<br>

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