高圧力下における蛋白質結晶表面の分子ステップのその場観察

DOI Web Site 参考文献16件

書誌事項

タイトル別名
  • In Situ Observation of Molecular Steps on the Surface of Protein Crystal under High Pressure
  • コウアツリョクカ ニ オケル タンパクシツ ケッショウ ヒョウメン ノ ブンシ ステップ ノ ソノバ カンサツ

この論文をさがす

説明

Velocities of elementary steps and two-dimensional nucleation rates on the {101} face of glucose isomerase (GI) crystals were measured at pressures of 0.1, 25 and 50 MPa. We conducted in situ observation of the steps by using a laser confocal microscope combined with differential interference contrast microscope (LCM-DIM) and high-pressure vessel with a sapphire window (t = 1 mm). Both step velocities and 2D nucleation rates increased with increasing pressure at T = 26.4°C and C = 5.6 mg ml-1. Solubilities Ce at high pressures were also measured by in situ observation of the moving steps around equilibrium temperatures. The solubility decreased with increasing pressure.

収録刊行物

参考文献 (16)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ