In Situ Observation of Molecular Steps on the Surface of Protein Crystal under High Pressure

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  • 高圧力下における蛋白質結晶表面の分子ステップのその場観察
  • コウアツリョクカ ニ オケル タンパクシツ ケッショウ ヒョウメン ノ ブンシ ステップ ノ ソノバ カンサツ

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Abstract

Velocities of elementary steps and two-dimensional nucleation rates on the {101} face of glucose isomerase (GI) crystals were measured at pressures of 0.1, 25 and 50 MPa. We conducted in situ observation of the steps by using a laser confocal microscope combined with differential interference contrast microscope (LCM-DIM) and high-pressure vessel with a sapphire window (t = 1 mm). Both step velocities and 2D nucleation rates increased with increasing pressure at T = 26.4°C and C = 5.6 mg ml-1. Solubilities Ce at high pressures were also measured by in situ observation of the moving steps around equilibrium temperatures. The solubility decreased with increasing pressure.

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