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- TANAKA Hideaki
- Mitsubishi-kasei Institute of Life Sciences
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- HONDA Koichi
- National Chemical Laboratory for Industry
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- UCHIDA Tsuneko
- Mitsubishi-kasei Institute of Life Sciences
Bibliographic Information
- Other Title
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- RNAオリゴマーの酵素的合成
- RNA オリゴマー ノ コウソテキ ゴウセイ
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Abstract
In order to systematically synthesize oligoribonucleotides with defined sequence, trimer unit having different bloking groups at 3'-hydroxyl and 5'-phosphate groups were enzymatically synthesizd. Deblocking at 3'- or 5'-end of the trimer unit gives an acceptor or a donor for the T4RNA ligase, respectively. For the blocking of the 3'-end, o-nitrobenzyl group was attached to the 2'-hydroxyl group, while for the blocking of 5'-phosphate, anilidate was employed. The f ormer was deblocked by photo-irradiation and the latter was by an acid. The hexamer produced by the ligation of the acceptor and the donoralso has the same blocking groups at 3'- and 5'-ends, and is capable to be utilized for the next synthetic reaction as an acceptor or a donor for T4RNA ligase.
Journal
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- NIPPON KAGAKU KAISHI
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NIPPON KAGAKU KAISHI 1983 (9), 1353-1357, 1983-09-10
The Chemical Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390001204389269376
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- NII Article ID
- 130004157648
- 40002843422
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- NII Book ID
- AN00186595
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- ISSN
- 21850925
- 03694577
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- NDL BIB ID
- 2604827
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed