Tissue Inhibitors of Metalloproteinases (TIMP-1 and TIMP-2) Stimulate Osteoclast Differentiation
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- Sobue Takanori
- Departments of Periodontology, Aichi-Gakuin University, School of Dentistry
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- Oguchi Masayo
- Departments of Periodontology, Aichi-Gakuin University, School of Dentistry
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- Tanaka Shigehisa
- Departments of Periodontology, Aichi-Gakuin University, School of Dentistry
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- Koide Masanori
- Departments of Periodontology, Aichi-Gakuin University, School of Dentistry
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- Ishihara Yuichi
- Departments of Periodontology, Aichi-Gakuin University, School of Dentistry
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- Yamashita Kyoko
- Biochemistry, Aichi-Gakuin University, School of Dentistry
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- Hayakawa Hirohisa
- Departments of Periodontology, Aichi-Gakuin University, School of Dentistry
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- Hayakawa Taro
- Biochemistry, Aichi-Gakuin University, School of Dentistry
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- Noguchi Toshihide
- Departments of Periodontology, Aichi-Gakuin University, School of Dentistry
Bibliographic Information
- Other Title
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- TIMP‐1およびTIMP‐2は破骨細胞形成を促進する
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Description
Tissue inhibitor of metalloproteinases-1 (TIMP-1) and TIMP-2, originally known as intrinsic inhibitors of matrix metalloproteinases (MMPs), are now known as constitutive components in serum and have potent cell growth-promoting activity. In this study, we examined the effects of TIMPs in FCS on osteoclast-like (OCL) cell formation in mouse bone marrow culture. The OCL cell formation is totally dependent on the presence of PGE2 or 1α25 (OH)2D3 or IL-1α. The effects of these factors were, however, not completely but greatly suppressed by deleting TIMP-1 and TIMP-2 from FCS in culture. But the suppression was almost fully recovered by the readdition of the same amount of recombinant (r) TIMPs which were detected in 10% FCS. As low molecular weight MMP inhibitors could not substitute rTIMPs for the recovery, TIMP activity on the OCL cell formation seemed to be independent from their MMP inhibitory activity. These results strongly suggest a secondary but potent stimulating activity of TIMPs on OCL cell formation. In conclusion, TIMPs in serum play an important role in osteoclastgenesis in mouse bone marrow culture.
Journal
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- Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology)
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Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) 47 (4), 297-307, 2005
JAPANESE SOCIETY OF PERIODONTOLOGY
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Details 詳細情報について
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- CRID
- 1390001204410513664
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- NII Article ID
- 10016919077
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- NII Book ID
- AN0019129X
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- ISSN
- 1880408X
- 03850110
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed