Relationship between Calcium Binding Proteins Associated with Mineralized Bone Nodule and 5'-Methylthioadenosine Metabolism in Cultured Rat Calvaria Cells.

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  • KASAHARA Shinji
    Department of Periodontology and Endodontology, Tokushima University School of Dentistry
  • KASAHARA Chika
    Department of Periodontology and Endodontology, Tokushima University School of Dentistry
  • NISHIKAWA Seiji
    Department of Periodontology and Endodontology, Tokushima University School of Dentistry
  • YAMAUCHI Noriyuki
    Department of Periodontology and Endodontology, Tokushima University School of Dentistry
  • OHISHI Keiji
    Department of Periodontology and Endodontology, Tokushima University School of Dentistry
  • KAHO Keiko
    Department of Periodontology and Endodontology, Tokushima University School of Dentistry
  • NAGATA Toshihiko
    Department of Periodontology and Endodontology, Tokushima University School of Dentistry
  • ISHIDA Hiroshi
    Department of Periodontology and Endodontology, Tokushima University School of Dentistry
  • WAKANO Yoichi
    Department of Periodontology and Endodontology, Tokushima University School of Dentistry

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Other Title
  • ラット頭蓋冠由来細胞が合成分泌するカルシウム結合性蛋白とメチルチオアデノシン代謝との関連

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5'-Methylthioadenosine (MTA) is formed during the biosynthesis of polyamines. We previously reported that 5'-difluoromethylthioadenosine (DFMTA), a synthetic inhibitor of MTA phosphorylase, caused intracellular adenine depletion and inhibited both alkaline phosphatase activity and bone nodule formation in cultured rat calvaria cells. In addition, exogenous adenine enhanced these two markers and partially reversed the DFMTA-induced inhibition in this culture system.<BR>Osteopontin, a non-collagenous protein associated with matrix mineralization, has been reported to be predominantly incorporated into bone nodules in concert with the progresism of in vitro mineralization on rat calvaria cells. In this study, we examined the effects of adenine and DFMTA on osteopontin contents incorporated into mineralized nodules by using polyacrylamide gel electrophoresis and `Stains-All` staining. Adenine increased the accumulation of osteopontin into mineralized nodule whereas DFMTA inhibited it. Furthermore the osteopontin contents which had been decreased by DFMTA recovered with the addition of adenine. These results suggest that adenine formed from MTA is important in the mineralization process of rat calvaria cells.

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