歯周病原因菌由来内毒素のヒト培養歯肉線維芽細胞のフィブロネクチン代謝に対する影響について

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  • Effect of LPS on Fibronectin Synthesis in Cultured Human Gingival Fibroblasts.

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The effect of lipopolysaccharides (LPS) on the production of fibronectin by cultured fibroblasts was studied. Fibroblasts were obtained from healthy and inflamed gingiva. The Gin-1 cell line was also tested. LPS were extracted from Bacteroides gingivalis (Porphyromonas gingivalis) 381 and Bacteroides intermedius (Prevotella intermedia) ATCC 33563 by the hot phenol-water method. The LPS and commercially available LPS from Escherichia coli (B 4: O 111) were added to the fibroblast cultures. Fibronectin levels on the cell surface and in the culture medium were measured periodically for 48 hours using ELISA. Fibronectin levels were expressed as . μg/μg protein. The following results were obtained: 1) The levels of fibronectin in the culture medium increased linearly with time in all dishes. There were no differences in fibronectin levels in the same cells whether LPS were added to the culture medium or not. 2) The profiles of cell surface fibronectin levels differed from dish to dish as did the time to reach the maximum level. Healthy gingival fibroblasts and Gin-1 produced more cell-associated fibronectins upon stimulation with LPS from B. gingivalis and B. intermedius, whereas inflamed gingival fibroblasts produced more fibronectins in response to LPS from B. gingivalis. 3) The total amounts of fibronectin on the cell surface and in the medium were greator in the inflamed cells_

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