歯周疾患病原菌由来内毒素によるＧｉｎ‐１　ｆｉｂｒｏｂｌａｓｔの代謝変動

DOI PubMed

書誌事項

タイトル別名
  • Metabolic alteration of Gin-1 fibroblast by lipopolysaccharide from periodontopathic bacteria.

この論文をさがす

抄録

Lipopolysaccharide (LPS) prepared from gramnegative bacteria including (1) Bacteroides gingivalis (381), (2) B. intermedius (20-3), (3) B. melaninogenicus (ATCC 15930), (4) Actinobacillus actinomycetemcomitans (Y-4), (5) Capnocytophaga species (M-12), (6) Eikenella corrodens (1073) and (7) Fusobacterium nucleatum (ATCC 25586) were extracted according to Westphal's hot phenol water method. A commercially available LPSs from (8) Escherichia coli 0127: B 8 and (9) Salmonella thyphimurium were also used for this study.<BR>Metabolic effects of these endotoxins upon normal human gingival fibroblast (Gin-1) were assessed by measuring the activity of glucose consumption and glycolytic enzymes in vitro.<BR>Each of the LPSs showed inhibition of glucose consumption to various degrees in a dose-responsive manner. The concentration of LPS for 50 percent inhibition (IC50) was calculated as 280. Eg/ml for C. species and F. nucleatum which showed intensive toxicity compared to that of B. intermedius, A. actinomycetemcomitans, E. coli and S. thyphimurium.<BR>The LPS prepared from periodontopathic bacteria at a concentration of 10. Eg/ml altered the enzymatic activity of malate dehydrogenase (MDH), glucose-6-phosphate dehydrogenase (G-6-P DH), pyruvate kinase (PK), lactate dehydrogenase (LDH) in various degrees without remarkable changes in morphology or viability of the cells.

収録刊行物

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ