Influence of cigarette smoke and nicotine on the morphological change of human periodontal ligament fibroblasts.
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- Nishiguchi Eiko
- Department of Dental Hygiene, Shonan Junior College
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- Suzuki Yukie
- Department of Dental Hygiene, Shonan Junior College
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- Kaneko Kazumi
- Department of Dental Hygiene, Shonan Junior College
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- Todoki Kazuo
- Department of Dental Hygiene, Shonan Junior College
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- Masumizu Toshiki
- Faculty of Pharmaceutical Sciences, Sojo University
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- Tsukinoki Keiichi
- Department of Maxillofacial diagnostic sience, division of Pathology, Kanagawa Dental College
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- Kawase Toshio
- Department of Dental Bioengineering, Kanagawa Dental College
Bibliographic Information
- Other Title
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- 歯根膜線維芽細胞の形態変化におよぼすたばこ煙とニコチンの影響
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Description
Cigarette smoking is a risk factor for periodontal disease. The effect of cigarette smoke and nicotine, a major component of tobacco, on the morphological changes of human periodontal ligament fibroblasts (HPLF) was investigated.When suspended HPLF were incubated with cigarette smoke and nicotine solution in a 5% CO2 atmosphere at 37°C, the cells did not neither adhered to the glass nor multiplied.When confluent HPLF were incubated with cigarette smoke and nicotine, shrinking of the cells, with the formation of intracellular spaces, and cellular lysis were observed. The changes depended on the nicotine concentrations and incubation periods. The expression of nicotine-induced cytokines was examined with a RayBioTM human Cytokine Kit using a chemiluminescence method. IL-6 and IL-8, both inflammatory cytokines, were expressed in the HPLF at a low concentration of nicotine. Cell death was observed in the presence of nicotine, as detected by TUNEL. Apoptosis appeared when the cells were incubated with a high concentration of nicotine. The generation of free radicals in the cigarette smoke and nicotine solutions was measured by the spin trapping technique using an electron spin resonance spectrometer(ESR) to identify superoxide anions and hydroxyl radicals. When the HPLF were incubated with hexamethonium, an inhibitor of nicotine receptors, the cells showed altered morphology as well as nicotine-induced morphological changes.<BR>These results suggest that the HPLF were destroyed via a direct local action through the generation of free radicals induced by nicotine.<BR>Nihon Shishubyo Gakkai Kaishi (J Jpn Soc Periodontol) 50(4) : 238-249, 2008.
Journal
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- Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology)
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Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) 50 (4), 238-249, 2008
JAPANESE SOCIETY OF PERIODONTOLOGY
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Details 詳細情報について
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- CRID
- 1390001204412061056
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- NII Article ID
- 10027095183
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- NII Book ID
- AN0019129X
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- ISSN
- 1880408X
- 03850110
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed