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  • A study of lipopolysaccharide derived from Bacteroides gingivalis.

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It has been supposed that lipopolysaccharide (LPS) derived from the gram negative bacteria of subgingival plaque is one of the important etiologic factors in periodontal disease.<BR>The purpose of this study was to detect specifically the LPS from Bacteroides gingivalis (Bg) and to determine the effects of gram-positive bacteria on LPS in culture supernatant of Bg.<BR>Enzyme-linked immunosorbent assay was used for specific detection of LPS from Bg. LPS of Bg could be measured in concentrations as low as 4 μg/ml.<BR>LPS of Bg was not detected in gingival crevicular fluid from periodontal disease patients.<BR>There were no significant differences in the concentration of LPS between the culture supernatant of Bg and co-cultivation of Bg and gram-positive bacteria.<BR>In this stuay, gram-positive bactria had no effects on release and degradation of LPS in the culture supernatant of Bg.

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