Interleukin-1.ALPHA. and interleukin-1.BETA. induction-capacity of endotoxin from periodontopathic bacteria in human peripheral monocytes and human gingival fibroblasts.

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  • 歯周病関連由来内毒素によるヒト末梢血単球及びヒト歯肉線維芽細胞に対するインターロイキン１α，インターロイキン１βの誘導能について

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Abstract

The present study was performed to detect interleukin-1α (IL-1α) and interleukin-1β (IL-1β) induction-capacity of human peripheral monocytes and normal human gingival fibroblasts stimulated with endotoxin from periodontopathic bacteria. Endotoxins were extracted from Bacteroides gingivalis 381 and Bacteroides intermedius ATCC 33563 using the hot phenol-water method. A commercially available endotoxin from Escherichia coli 0111: B 4 was also used. Monocytes isolated from human peripheral blood were stimulated with RPMI 1640 medium containing 5% FBS and antibiotics with the addition of endotoxins in various concentrations (0, 0. 1, 1 and 10 μg/ml). Human gingival fibroblasts (Gin-1 ATCC . E CRL 1292) were maintained using DMEM containing 10% FBS and antibiotics. When the fibroblasts became confluent they were stimulated with each of the endotoxins at various concentrations (0, 5, 10 and 20. μg/ml). IL-α . and IL-1β induction-capacity of peripheral monocytes and gingival fibroblasts stimulated with endotoxins was assessed using the enzymelinked immunosorbent assay procedure. The results were as follows:<BR>Monocyte IL-1α activity increased within 36 hours of exposure to various concentrations of each endotoxin. Monocyte IL-1β activity appeared to reach a plateau 24 hours after stimulation with the endotoxins of Bacteroides intermedius and E. coli but tended to increase slightly within 36 hours after stimulation with endotoxin from Bacteroides gingivalis. IL-1α and IL-1β induction-capacity varied with changes in the concentration of the various endotoxins. Gingival fibroblast IL-1α and IL-1β activity was undetectable up to 96 hours after stimulation with the various concentrations of the endotoxins tested.

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