書誌事項
- タイトル別名
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- Spheroid Formation by Human Periodontium-Derived Cells. Characterization of Human Periodontal Ligament Fibroblast(HPLF) in Spheroid Formation.
- Characterization of Human Periodontal Ligament Fibroblast (HPLF) in Spheroid Formation
- スフェロイドにおけるヒト歯根膜由来線維芽細胞 (HPLF) の特徴について
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抄録
We previously reported that periodontium-derived cells could form spheroids and that the spheroids could be used for periodontal reconstructive therapy because they contained cells with proliferative activity and which produce extracellular matrix. In this study, we performed an immunohistochemical study on spheroids formed by human periodontal ligament fibroblasts (HPLFs), which play an important role in periodontal reconstruction, using the antibodies against osteocalcin (OC) and fibroblast growth factor (FGF). The results showed that HPLF could produce OC in the spheroid environment. Much more OC expression was observed on day 40 after spheroid formation, than on day 7. FGFs, which maintain tissue construction, and growth factor for mesenchymal cells, were also observed. We investigated the viability of the cells in the spheroids by using a fluorescent material fluorescein diacetate-propidium iodide; FD (A-PI), and found that almost all cells survived and that no dead colonies were observed. The finding that, HPLF in spheroids can express OC in vitro, and that OC expression appears to be affected by the spheroid environment. FGFs were also contained in the spheroids, so spheroids may be capable of being applied to periodontal reconstructive therapy.
収録刊行物
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- 日本歯周病学会会誌
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日本歯周病学会会誌 37 (4), 650-657, 1995
特定非営利活動法人 日本歯周病学会
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詳細情報 詳細情報について
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- CRID
- 1390001204413220736
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- NII論文ID
- 110004726121
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- NII書誌ID
- AN0019129X
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- ISSN
- 1880408X
- 03850110
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可