Evaluation of the penetration ability and antimicrobial efficacy of Listerine<sup>® </sup>Natural Care against <i>Streptococcus mutans</i> biofilms

DOI 38 References Open Access
  • Ohsumi Tatsuya
    Division of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences
  • Takenaka Shoji
    Division of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences
  • Sakaue Yuuki
    Division of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences
  • Wakamatsu Rika
    Division of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences
  • Terao Yutaka
    Division of Microbiology and Infectious Diseases, Department of Oral Health Science, Niigata University
  • Ohshima Hayato
    Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences
  • Okiji Takashi
    Division of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences

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  • <i>Streptococcus mutans</i>バイオフィルムに対するリステリン<sup>®</sup>ナチュラルケアの浸透性と殺菌効果の評価

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The aims of this study were to evaluate the penetration ability and antimicrobial efficacy of an alcohol-free mouth rinse (Listerine® Natural Care; N), newly developed to reduce the irritative property and improve the mouthfeel of Listerine®, using artificially developed Streptococcus mutans biofilms. As test mouth rinses, Listerine® Zero (Z), Listerine® Fresh Mint (F) and an alcohol-free chlorhexidine gluconate-containing mouth rinse (CHG) were investigated. S.mutans biofilms were grown on glass-based dishes for 24 h under anaerobic conditions. The ability of the mouth rinses to penetrate these biofilms were analyzed by time-lapse monitoring of the fluorescence loss of the calcein-AM-stained biofilms by confocal laser scanning microscopy. The antimicrobial effects of the mouth rinses were evaluated by colony counting and Live/Dead staining observation. For each of the mouth rinses evaluated, a high correlation was observed between the time required to reach 50% of the initial fluorescence intensity and the biofilm thickness. The penetration velocity of N was similar to that of Z and F and significantly higher than that of CHG. For 30s exposure, the number of viable cells after exposure to N was similar to that after exposure to Z and F. Live/Dead staining observation showed that 99% of the cells were propidium iodide-positive after exposure to any of N, Z and F, being significantly higher than the percentage of propidium iodide-positive cells found after exposure to CHG. In the present study, it was concluded that the penetration ability and antimicrobial efficacy of Listerine® Natural Care were similar to those of Listerine® Zero and Listerine® Fresh Mint, and significantly superior to those of the chlorhexidine gluconate-containing mouth rinse. Nihon Shishubyo Gakkai Kaishi (J Jpn Soc Periodontol) 56(3)：291-301, 2014.

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