書誌事項
- タイトル別名
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- A Transgenic Plant that Detects Estrogenic Activity
- ジョセイ ホルモン オ ミツケル ショクブツ
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抄録
A multitude of physiological processes are regulated by the endocrine system in animals. Endocrine-disrupting chemicals (EDCs) can potentially affect physiological processes of an organism by binding to steroid hormone receptors. Therefore, it is necessary to develop methods that can detect the presence of and effectively remove EDCs from the environment. We have developed an easy-to-use and inexpensive reporter gene assay system using transgenic Arabidopsis thaliana that can detect estrogenic activity. This transgenic plant constantly expresses two effector proteins, chimeric estrogen receptor and chimeric co-activator, in its nuclear. Estrogen-dependent interaction between the two effectors cause transcriptional activation of reporter gene, β-glucuronidase (GUS) . This transgenic plant has the capability to detect the existence of 17 β-estradiol at a concentration of 50 pM (13 pg/mL) in agar medium. The plant could also detect other estrogen-like substances like diethylstilbestrol, p-n-nonylphenol, bisphenol A, and genistein.
収録刊行物
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- 廃棄物学会誌
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廃棄物学会誌 15 (5), 247-253, 2004
一般社団法人 廃棄物資源循環学会
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詳細情報 詳細情報について
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- CRID
- 1390001204415790336
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- NII論文ID
- 10013394495
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- NII書誌ID
- AN10414674
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- ISSN
- 18838960
- 09170855
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- NDL書誌ID
- 7127946
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
