Monitoring of microorganisms by using the marker gene

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  • NODA Naohiro
    Institute for Biological Resources and Functions, National Research Institute of Advanced Industrial Science and Technology (AIST)
  • NAKAMURA Kazunori
    Institute for Biological Resources and Functions, National Research Institute of Advanced Industrial Science and Technology (AIST)

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Other Title
  • マーカー遺伝子を用いた微生物のモニタリング
  • マーカー イデンシ オ モチイタ ビセイブツ ノ モニタリング

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Description

It would be highly desirable that we are able to estimate the bio-safety risks of the genetically modified microorganisms (GMM) for human health and the biological diversity with regard to the use of GMM. Thus, specific microorganisms that are used for bioremediation should be monitored; further, the safety of these microorganisms should be estimated. We developed technologies that enabled labeling of specific microorganisms using the marker gene and monitoring of the microbial community structure in the environment in which the specific microorganisms were introduced. We substituted certain nucleic acids without changing their amino acid sequences and obtained bphCm that had the same activity when compared with wild type bphC gene. Subsequently, the GMM (P putida KTTG39) possessing green and red fluorescence proteins and bphCm gene were constructed. The genetically engineered P putida KTTG39 was inoculated into the activated sludge which was employed as the model ecological system. The outcomes of the inoculated P putida and the microbial community structure could be estimated by using real-time quantitative Q-probe PCR and PCR-DGGE, respectively. In conclusion, we demonstrated that the combined use of the GMM and the mo lecular technologies were useful for evaluating the impact of the GMM in the natural environment.

Journal

  • ENVIRONMENTAL SCIENCE

    ENVIRONMENTAL SCIENCE 20 (5), 371-380, 2007

    SOCIETY OF ENVIRONMENTAL SCIENCE, JAPAN

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