68. Cyanamide-biosynthesizing organ in hairy vetch Vicia villosa subsp. varia
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- Kasahara Ryohei
- Faculty of Agriculture, Shinshu University
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- Kamo Tsunashi
- Faculty of Agriculture, Shinshu University
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- Hirota Mitsuru
- Faculty of Agriculture, Shinshu University
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- Hiradate Syuntaro
- National Institute for Agro-Environmental Sciences
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- Yamaya Hiroko
- National Institute for Agro-Environmental Sciences
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- Fujii Yoshiharu
- National Institute for Agro-Environmental Sciences
Bibliographic Information
- Other Title
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- 68.ヘアリーベッチにおけるシアナミドの生合成部位(口頭発表)
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Abstract
Cyanamide (NH_2CN) has been isolated from hairy vetch Vicia villosa subsp. varia as a natural product for the first time. We have already clarified that the cyanamide content in hairy vetch seedlings cultivated in the growth chamber reaches its maximum at 3-4 week. The present study aims at elucidating the organ in which cyanamide is biosynthesized. The cyanamide contents in the leaves, the stems, and the roots of 4-week-old seedlings were 629 ± 209, 256 ± 134, and < 20 μg/gFW (average ± SD), respectively. This suggests that cyanamide is biosynthesized in the aerial parts (the leaves plus the stems). To investigate the cyanamide-biosynthesizing organ, we compared the ^^<15>N incorporation into cyanamide in the whole aerial parts to that in the apical portion including the younger leaves. The ^^<15>N isotope abundance ratio in the isolated cyanamide was 3.99 ± 0.61% (average ± SE) when the whole aerial parts was incubated with ^^<15>N-labeled nitrogen sources, which was significantly higher than that of control (0.28 ± 0.03%). In contrast, the cyanamide isolated from the apical portion was not ^^<15>N-labeled on the same condition. These results imply that cyanamide is biosynthesized in the mature leaves.
Journal
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- The Janapese Society for Chemical Regulation of Plants, Abstract
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The Janapese Society for Chemical Regulation of Plants, Abstract 42 (0), 81-, 2007
The Janapese Society for Chemical Regulation of Plants
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Details 詳細情報について
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- CRID
- 1390001204423356544
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- NII Article ID
- 110006474761
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- NII Book ID
- AA11550064
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- ISSN
- 21896313
- 09191887
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed