15.イネ2番染色体に存在するファイトアレキシン生合成遺伝子クラスター(口頭発表)
書誌事項
- タイトル別名
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- 15. A gene cluster on rice chromosome 2 that is involved in biosynthesis of diterpenoid phytoalexins
説明
Momilactones and phytocassanes are major diterpenoid phytoalexins, which are produced in elicitor-treated rice cells. Two cytochrome P450 monooxigenases (P450s) and a dehydrogenase whose genes are found to be clustered on chromosome 4, are involved in momilactone biosynthesis. Likewise, six P450 genes form a gene cluster on chromosome 2 with the diterpene cyclase genes OsCPS2 and OsKSL7 for phytocassane biosynthesis. However, functions of these P450s have not yet been clarified. Here, we report functional identification of two P450 genes, CYP71Z6 and CYP71Z7, of the six P450 genes organized in the gene cluster on chromosome 2. As CYP71Z6 and CYP71Z7 genes are highly homologous to each other, we attempted to generate their double knockdown RNAi lines. The CYP71Z6/CYP71Z7 double knockdown suppressed the elicitor-inducible production of phytocassanes A, B, and D, suggesting that either or both of CYP71Z6 and CYP71Z7 genes are required for hydroxylation at 2-position of the biosynthetic intermediates of phytocassane A, B, and D. These results provide strong evidence for the presence on chromosome 2 of a gene cluster involved in phytocassane biosynthesis.
収録刊行物
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- 植物化学調節学会 研究発表記録集
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植物化学調節学会 研究発表記録集 43 (0), 29-, 2008
一般社団法人 植物化学調節学会
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詳細情報 詳細情報について
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- CRID
- 1390001204423406592
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- NII論文ID
- 110007468189
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- ISSN
- 21896313
- 09191887
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
