書誌事項
- タイトル別名
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- Genotypic Characterization of Clostridium botulinum Strains Producing Type A Neurotoxin Complexes by Random Amplified Polymorphic DNA and PCR-RFLP Analysis
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抄録
In order to genotypically characterize Clostridium botulinum type A strains, we examined 23 strains from various origins (food-borne botulism, infant botulism, honey, honey-related materials and soil), by random amplified polymorphic DNA (RAPD) analysis and characterized the type A botulinum neurotoxin complex genes by PCR and PCR-RFLP analysis. By RAPD analysis, C. botulinum strains associated with infant botulism in Japan and a strain 804-1H, isolated from Brazilian honey, were clearly differentiated from the other strains examined.PCR and PCR-RFLP analysis were performed for the neurotoxin complex genes (type Aneurotoxin (BoNT/A), type B neurotoxin (BoNT/B), nontoxic-nonhemagglutinin (NTNH), hemaggulutinin (HA), and p47 protein). All of 23 strains tested gave positive amplification for BoNT/A and NTNH genes, and the PCR-RFLP analysis differentiated six strains associated with infant botulism in Japan, a strain from Brazilian honey and a strain AF84 (type 2) from other strains (type 1). For HA gene, the type 1 strains gave amplification while the type 2 strains gave no amplification. For BoNT/B gene, the type 1 strains (a strain Renkon and three American infant botulism strains) gave amplification (silent B strains). For p47 gene, the type 2 strains and the silent B strains were positive. The combinations of the PCR of HA, p47, and BoNT/B genes and the PCR-RFLP of BoNT/A, NTNH genes demonstrated three genotypes (A 1, A2, and A3) of neurotoxin complex genes. Thus, these PCR-based methods are useful for simple and rapid differentiation of the BoNT-producing C. botulinum strains.
収録刊行物
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- 日本食品微生物学会雑誌
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日本食品微生物学会雑誌 22 (4), 148-154, 2005
日本食品微生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001204425574016
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- NII論文ID
- 130004103566
- 10016965107
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- NII書誌ID
- AN10552871
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- ISSN
- 18825982
- 13408267
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- NDL書誌ID
- 7803851
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可