ＰＣＲ法による水，食肉および野菜中の大腸菌と大腸菌群の検出に関する検討

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  • Studies on the Detection of Escherichia coli and Coliform Bacteria in Water, Meat and Vegetables by the Polymerase Chain Reaction
  • PCRホウ ニ ヨル ミズ ショクニク オヨビ ヤサイ チュウ ノ ダイチョウキン ト ダイチョウキングン ノ ケンシュツ ニ カンスル ケントウ

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We applied the polymerase chain reaction (PCR) to the detection of Escherichia coli and coliform bacteria in water and food. Primer sets targeting the transferase gene (wecF), the β-galactosidase gene (lacZ) and the 16S rRNA gene (E. coli 16S rRNA) of E. coli were used in the PCR, and the following results were obtained.<BR>1) The frequencies of preservation of the wecF, lacZ and E. coli 16S rRNA genes were 12/12 (100%), 99/99 (100%) and 99/99 (100%), respectively, for E. coli from food and human feces, and 28/63 (44%), 70/177 (39%) and 0/177 (0%), respectively, for coliform bacteria including Klebsiella spp., Citrobacter spp. and others from food.<BR>2) The detection frequencies of the wecF, lac and E. coli 16S rRNA genes from the BGLB culture of 69 samples of food (23 samples of meat and 46 samples of vegetables) were 73%, 65% and 37%, respectively. A significant correlation was found between the isolation of E. coli and the detection of the E. coli 16S rRNA gene, but no correlation was found between the isolation of coliform bacteria and the detection of the E . coli 16S rRNA gene. Also, no significant correlation was found between the isolation of coliform bacteria and the detection of the wecF or lacZgene.<BR>3) A significant correlation between the detection of E. coli 16S rRNA and the isolation of E. coli was observed, when fecal contamination of 153 water samples from various sources of water supply were examined by the defined substrate method using colilert.<BR>These results show that PCR targeting the E. coli 16S rRNA gene is useful for rapid and specific detection of E. coli in water and food.

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